Coding

Part:BBa_K2045002:Design

Designed by: Thiban Navaratnarajah   Group: iGEM16_Concordia   (2016-10-14)


fhuA:Ferrichrome Iron outer membrane transporter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 682
    Illegal AgeI site found at 820
    Illegal AgeI site found at 1225
    Illegal AgeI site found at 1999
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 724


Design Notes

It was imoportant to consider the C and N terminals in order to ensure that we know which terminal is placed on the extracellular extremities of the cell.


Source

iGEM BCCS-Bristol’s part, BBa_K259000.

References